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Image Search Results
Journal: Cellular and Molecular Bioengineering
Article Title: Podoplanin is Responsible for the Distinct Blood and Lymphatic Capillaries
doi: 10.1007/s12195-022-00730-2
Figure Lengend Snippet: BEC and LEC form distinct cord-like structures on 2D Matrigel and 3D fibrin gel assays. (a) BEC (pre-labeled in pre-labeled in CellTracker™ Red CMTPX) and LEC (pre-labeled in CellTracker™ Green CMFDA) were seeded on 2D Matrigel at ratios of 100:0, 80:20, 50:50, 20:80, and 0:100 (BECs:LECs). Representative images of cord-like structures (CLS) formation were imaged at 12 h. Scale bars are 500 μ m. (b) BEC and LEC were encapsulated in 3D fibrin gel assay. From left to right, red-BEC only, red-BEC and green-BEC (50:50), red-BEC and green-LEC (50:50), red-LEC and green-LEC (50:50), and green-LEC only. Representative images of CLS formation were imaged at 48 h. Scale bars are 500 μ m. CLS formed on 3D fibrin gel was quantified for tube length using AutoTube for (c) BEC and (d) LEC. Data represents mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\pm$$\end{document} ± SD, n = 4 per group, n.s. p > 0.05, * p < 0.05. All P values were determined by unpaired t tests. (e) An equal density of BEC or LEC (5 × 10 6 cells/mL) were seeded on each chamber of the IdenTx Chips separated by the fibrin gels in the middle. Representative images of BEC or LEC sprouting into the fibrin gels after 48 h of culture. Scale bars are 500 μ m.
Article Snippet: Analysis was performed using
Techniques: Labeling
Journal: Cellular and Molecular Bioengineering
Article Title: Podoplanin is Responsible for the Distinct Blood and Lymphatic Capillaries
doi: 10.1007/s12195-022-00730-2
Figure Lengend Snippet: The roles of FLCN and PDPN on cord-like networks formation. (a and b) Luciferase knockout BEC (rBEC ΔLuc ) or (c and d) FLCN knockout BEC (rBEC ΔFLCN ) stained in red are encapsulated in 3D fibrin gels together with (a and c) Luciferase knockout LEC (gLEC ΔLuc ) or (b and d) PDPN knockout LEC (rLEC ΔPDPN ) stained in green. Representative images of CLS formation were imaged at 48 h. Scale bars are 500 μ m. (e and f) CLS formed on 3D fibrin gel was quantified for tube length using AutoTube. Data represents mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\pm$$\end{document} ± SD, n = 4 per group, n.s. p > 0.05, * p < 0.05. All p values were determined by ANOVA followed by post hoc testing with Tukey analysis.
Article Snippet: Analysis was performed using
Techniques: Luciferase, Knock-Out, Staining